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Primer sequence disclosure: a clarification of the MIQE guidelines.
Bustin SA, Benes V, Garson JA, Hellemans J, Huggett J, Kubista M, Mueller R, Nolan T, Pfaffl MW, Shipley GL, Vandesompele J, Wittwer CT. Bustin SA, et al. Among authors: pfaffl mw. Clin Chem. 2011 Jun;57(6):919-21. doi: 10.1373/clinchem.2011.162958. Epub 2011 Mar 18. Clin Chem. 2011. PMID: 21421813 Free article. No abstract available.
The need for transparency and good practices in the qPCR literature.
Bustin SA, Benes V, Garson J, Hellemans J, Huggett J, Kubista M, Mueller R, Nolan T, Pfaffl MW, Shipley G, Wittwer CT, Schjerling P, Day PJ, Abreu M, Aguado B, Beaulieu JF, Beckers A, Bogaert S, Browne JA, Carrasco-Ramiro F, Ceelen L, Ciborowski K, Cornillie P, Coulon S, Cuypers A, De Brouwer S, De Ceuninck L, De Craene J, De Naeyer H, De Spiegelaere W, Deckers K, Dheedene A, Durinck K, Ferreira-Teixeira M, Fieuw A, Gallup JM, Gonzalo-Flores S, Goossens K, Heindryckx F, Herring E, Hoenicka H, Icardi L, Jaggi R, Javad F, Karampelias M, Kibenge F, Kibenge M, Kumps C, Lambertz I, Lammens T, Markey A, Messiaen P, Mets E, Morais S, Mudarra-Rubio A, Nakiwala J, Nelis H, Olsvik PA, Pérez-Novo C, Plusquin M, Remans T, Rihani A, Rodrigues-Santos P, Rondou P, Sanders R, Schmidt-Bleek K, Skovgaard K, Smeets K, Tabera L, Toegel S, Van Acker T, Van den Broeck W, Van der Meulen J, Van Gele M, Van Peer G, Van Poucke M, Van Roy N, Vergult S, Wauman J, Tshuikina-Wiklander M, Willems E, Zaccara S, Zeka F, Vandesompele J. Bustin SA, et al. Among authors: pfaffl mw. Nat Methods. 2013 Nov;10(11):1063-7. doi: 10.1038/nmeth.2697. Nat Methods. 2013. PMID: 24173381
Cautionary Note on Contamination of Reagents Used for Molecular Detection of SARS-CoV-2.
Huggett JF, Benes V, Bustin SA, Garson JA, Harris K, Kammel M, Kubista M, McHugh TD, Moran-Gilad J, Nolan T, Pfaffl MW, Salit M, Shipley G, Vallone PM, Vandesompele J, Wittwer C, Zeichhardt H. Huggett JF, et al. Among authors: pfaffl mw. Clin Chem. 2020 Nov 1;66(11):1369-1372. doi: 10.1093/clinchem/hvaa214. Clin Chem. 2020. PMID: 32894756 Free PMC article.
Disclosing quantitative RT-PCR raw data during manuscript submission: a call for action.
Untergasser A, Hellemans J, Pfaffl MW, Ruijter JM, van den Hoff MJB, Dragomir MP, Adamoski D, Dias SMG, Reis RM, Ferracin M, Dias-Neto E, Marsh I, Kubista M, Fabbri M, Goel A, Slabý O, Knutsen E, Chen B, Negrini M, Mimori K, Pichler M, Papatriantafyllou M, Anfossi S, Schmittgen TD, Huggett J, Bustin S, Vandesompele J, Calin GA; HEROIC (tHe initiativE gRoup On qRT dIsClosure) Consortium. Untergasser A, et al. Among authors: pfaffl mw. Mol Oncol. 2023 May;17(5):713-717. doi: 10.1002/1878-0261.13418. Epub 2023 Mar 27. Mol Oncol. 2023. PMID: 36916500 Free PMC article.
219 results