Laser-based microscopic approaches: application to cell signaling in environmental lung disease
Biotechniques. 2001 Oct;31(4):880-2, 884, 886-8, 890, 892-4.
doi: 10.2144/01314rv01.
Affiliation
- 1 Department of Pathology and Microscopy Imaging Center, University of Vermont, Burlington 05405, USA. dtaatjes@zoo.uvm.edu
Abstract
Cell-imaging approaches using new laser-based technologies have a wide applicability to thefields of pathology and cell biology. Here, we present the application of several of these techniques, including confocal scanning laser microscopy (CSLM), laser scanning cytometry (LSC), and laser capture microdissection (LCM), to studies of cell signaling by environmental agents in lung disease. Using both cells in culture and lung tissue, we show that these technologies are powerful tools for understanding signal transduction cascades elicited by toxic agents, such as oxidants and asbestosfibers, and their relationship to the development of cell injury and proliferation, responses leading to lung disease and/or repair.
Publication types
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Research Support, U.S. Gov't, P.H.S.
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Review
MeSH terms
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Air Pollutants / toxicity*
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Animals
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Biotechnology
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Cell Communication
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Flow Cytometry / methods
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Lasers
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Lung Diseases / etiology*
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Lung Diseases / pathology
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Lung Diseases / physiopathology*
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Male
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Mice
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Mice, Inbred C57BL
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Microscopy, Confocal / methods*
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Mitogen-Activated Protein Kinases / metabolism
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Polymerase Chain Reaction
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Signal Transduction
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Transfection
Substances
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Air Pollutants
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Mitogen-Activated Protein Kinases