Use of PCR to diagnose Toxoplasma gondii chorioretinitis in eyes with severe vitritis

Clin Exp Ophthalmol. 2005 Apr;33(2):184-7. doi: 10.1111/j.1442-9071.2005.00992.x.

Abstract

Two cases are reported of intraocular inflammation in which severe vitritis hampered the fundal view, making an accurate clinical diagnosis impossible, and vitreous analysis using conventional techniques was unhelpful. PCR for Toxoplasma gondii was positive in both cases and provided the only way of confirming the diagnosis. Other ocular samples also underwent PCR for Toxoplasma DNA and the specificity of this approach is demonstrated.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chorioretinitis / diagnosis*
  • Chorioretinitis / parasitology
  • DNA, Protozoan / analysis
  • Eye Diseases / diagnosis*
  • Eye Diseases / parasitology
  • Female
  • Humans
  • Middle Aged
  • Polymerase Chain Reaction / methods*
  • Toxoplasma / genetics
  • Toxoplasma / isolation & purification*
  • Toxoplasmosis / diagnosis*
  • Toxoplasmosis / parasitology
  • Vitreous Body / parasitology*

Substances

  • DNA, Protozoan