Interferon-beta antibodies have a higher affinity in patients with neutralizing antibodies compared to patients with non-neutralizing antibodies

J Neuroimmunol. 2006 May;174(1-2):174-9. doi: 10.1016/j.jneuroim.2006.01.017. Epub 2006 Mar 23.

Abstract

In this study, we investigated the affinity, determined by a relative affinity assay, using increasing concentrations of sodium-isothiocyanate to disrupt the antigen antibody binding of neutralizing and non-neutralizing antibodies against interferon-beta (IFNbeta)-1a and -1b in 73 serum samples of MS patients treated with IFNbeta-1a or -1b. Relative affinity values were significantly higher in NAB-positive compared to NAB-negative samples and in samples of IFNbeta-1a-treated patients compared to IFNbeta-1b. A significant positive correlation between relative affinity values and therapy duration indicates affinity maturation as another qualitative factor in IFNbeta neutralization.

Publication types

  • Clinical Trial
  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Analysis of Variance
  • Antibodies / physiology
  • Antibodies / therapeutic use*
  • Binding, Competitive / immunology
  • Cross Reactions
  • Enzyme-Linked Immunosorbent Assay / methods
  • Humans
  • Immunotherapy / methods
  • Interferon-beta / immunology*
  • Interferon-beta / metabolism
  • Multiple Sclerosis / immunology
  • Multiple Sclerosis / therapy*
  • Neutralization Tests / methods
  • Time Factors
  • Treatment Outcome

Substances

  • Antibodies
  • Interferon-beta