Impaired cutaneous T-cell attracting chemokine elevation and adipose-derived stromal cell migration in a high-glucose environment cause poor diabetic wound healing

Wei-Ting Wang; Su-Shin Lee; Ya-Chin Wang; Ya-Wei Lai; Yur-Ren Kuo; Yueh-Bih Tang Chen; Yu-Sen Liu; Yi-Chia Wu

doi:10.1016/j.kjms.2018.05.002

Impaired cutaneous T-cell attracting chemokine elevation and adipose-derived stromal cell migration in a high-glucose environment cause poor diabetic wound healing

Kaohsiung J Med Sci. 2018 Oct;34(10):539-546. doi: 10.1016/j.kjms.2018.05.002. Epub 2018 May 30.

Authors

Wei-Ting Wang¹, Su-Shin Lee², Ya-Chin Wang³, Ya-Wei Lai⁴, Yur-Ren Kuo⁵, Yueh-Bih Tang Chen⁶, Yu-Sen Liu³, Yi-Chia Wu⁷

Affiliations

¹ Center of Teaching and Research, Kaohsiung Municipal Ta-Tung Hospital, Kaohsiung, Taiwan.
² Division of Plastic Surgery, Department of Surgery, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan; Center of Stem Cell Research, Kaohsiung Medical University, Kaohsiung, Taiwan; Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.
³ Division of Plastic Surgery, Department of Surgery, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan.
⁴ Department of Plastic Surgery, Kaohsiung Municipal Ta-Tung Hospital, Kaohsiung, Taiwan.
⁵ Division of Plastic Surgery, Department of Surgery, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan; Center of Stem Cell Research, Kaohsiung Medical University, Kaohsiung, Taiwan; Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan; Graduate Institute of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.
⁶ Center for Human Appearance, Department of Plastic Surgery, Far-eastern Memorial Hospital, New Taipei City, Taiwan.
⁷ Division of Plastic Surgery, Department of Surgery, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan; Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan; Department of Plastic Surgery, Kaohsiung Municipal Ta-Tung Hospital, Kaohsiung, Taiwan; Ph.D. Program in Translational Medicine, Kaohsiung Medical University and Academia Sinica, Taipei, Taiwan. Electronic address: yichiawu1124@gmail.com.

PMID: 30309481
DOI: 10.1016/j.kjms.2018.05.002

Abstract

Diabetic wound care is a major health care concern. The major cause of non-healing of wounds in patients with diabetes mellitus (DM) patients mainly involves poor glycemic control, which hinders the migration of progenitor cells including mesenchymal stem cells to the wound site. In this study, we introduced adipose-derived stromal cells (ADSCs) into wound sites and demonstrated that the local transplantation of ADSCs accelerated DM-related wound healing. Furthermore, the migration ability of ADSCs, which diminishes in a high-glucose environment, was partially restored by the exogenous replenishment of the cutaneous T-cell attracting chemokine (CTACK/CCL27). Our findings suggest that CTACK is a potential novel therapeutic target in DM-related wound healing.

Keywords: ADSC; CTACK/CCL27; Diabetes mellitus; Wound.

MeSH terms

Adipose Tissue / cytology*
Animals
Blood Glucose / metabolism*
Cell Movement / physiology*
Cells, Cultured
Chemokine CCL27 / metabolism*
Diabetes Mellitus / blood*
Diabetes Mellitus / metabolism*
Humans
Male
Rats
Rats, Wistar
Stromal Cells / cytology*
Wound Healing / physiology*

Substances

Blood Glucose
Chemokine CCL27