Identification and functional characterization of a superoxide dismutase (CuZnSOD) from Pinctada fucata martensii

Zhijie Guo; Chaojie Li; Haiying Liang; Jiaping Zhu

doi:10.1016/j.fsi.2024.109599

Identification and functional characterization of a superoxide dismutase (CuZnSOD) from Pinctada fucata martensii

Fish Shellfish Immunol. 2024 May 1:149:109599. doi: 10.1016/j.fsi.2024.109599. Online ahead of print.

Authors

Zhijie Guo¹, Chaojie Li¹, Haiying Liang², Jiaping Zhu¹

Affiliations

¹ Fisheries College of Guangdong Ocean University, Zhanjiang, Guangdong, 524088, China.
² Fisheries College of Guangdong Ocean University, Zhanjiang, Guangdong, 524088, China; Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Zhanjiang, Guangdong, 524088, China. Electronic address: zjlianghy@126.com.

PMID: 38701990
DOI: 10.1016/j.fsi.2024.109599

Abstract

Copper/zinc superoxide dismutase (Cu/Zn-SOD) can effectively eliminate reactive oxygen species (ROS)，avoid damage from O₂ to the body, and maintain O₂ balance. In this study, multi-step high-performance liquid chromatography (HPLC), combined with Mass Spectrometry (MS), was used to isolate and identify Cu/Zn-SOD from the serum of Pinctada fucata martensii (P. f. martensii) and was designated as PmECSOD. With a length of 1864 bp and an open reading frame (ORF) of 1422 bp, the cDNA encodes a 473 amino acid protein. The PmECSOD transcript was detected in multiple tissues by quantitative real-time PCR (qRT-PCR), with its highest expression level being in the gills. Additionally, the temporal expression of PmECSOD mRNA in the hemolymph was highest at 48 h after in vivo stimulation with Escherichia coli and Micrococcus luteus. The results from this study provide a valuable base for further exploration of molluscan innate immunity and immune response.

Keywords: Cu/zn-SOD; HPLC; Pinctada fucata martensii; Quantitative real-time PCR.