Ganoderma lucidum extract attenuates corticotropin-releasing hormone-induced cellular senescence in human hair follicle cells

Sunhyoung Lee; So Young Kim; Seunghee Lee; Sunhyae Jang; Sungjoo Tommy Hwang; Youngji Kwon; Jaehwan Choi; Ohsang Kwon

doi:10.1016/j.isci.2024.109675

Ganoderma lucidum extract attenuates corticotropin-releasing hormone-induced cellular senescence in human hair follicle cells

iScience. 2024 Apr 6;27(5):109675. doi: 10.1016/j.isci.2024.109675. eCollection 2024 May 17.

Authors

Sunhyoung Lee^{1

2}, So Young Kim¹, Seunghee Lee^{1

2}, Sunhyae Jang¹, Sungjoo Tommy Hwang³, Youngji Kwon⁴, Jaehwan Choi⁴, Ohsang Kwon^{1

2}

Affiliations

¹ Department of Dermatology, Seoul National University College of Medicine, Laboratory of Cutaneous Aging and Hair Research, Biomedical Research Institute, Seoul National University Hospital, Institute of Human-Environment Interface Biology, Medical Research Center, Seoul National University, Seoul 03080, South Korea.
² Department of Biomedical Sciences, Seoul National University College of Medicine, Seoul 03080, South Korea.
³ Dr. Hwang's Hair-Hair Clinic, Seoul 06035, South Korea.
⁴ R&I Center, COSMAX BTI, Seongnam, Gyeonggi-do, South Korea.

Abstract

Corticotropin-releasing hormone (CRH) is a key mediator in stress-induced hair growth inhibition. Here, we investigated the impact of stress-induced senescence and evaluated the potential of Ganoderma lucidum (GL) extract in mitigating CRH-induced senescence in human hair follicle cells (hHFCs). We show that CRH treatment increased the senescence-associated beta-galactosidase (SA-β-GAL) activity and reactive oxygen species (ROS) formation in hHFCs and suppressed alkaline phosphatase (ALP) activity and anagen-inducing genes. However, GL extract restored ALP activity and decreased the expression levels of anagen-related genes in CRH-treated hHFCs. It decreased SA-β-GAL activity, reduced ROS production, and prevented the phosphorylation of MAPK signaling pathways in CRH-related stress response. Moreover, GL reversed the CRH-induced inhibition of two-cell assemblage (TCA) elongation and Ki67 expression. GL extract attenuates stress-induced hair follicular senescence by delaying catagen entry and scavenging ROS. Our findings suggest that GL extract could be used for treating stress-induced hair loss.

Keywords: Endocrinology; Molecular biology.