Complete mitochondrial genome data and phylogenetic analysis of Papilio macilentus Janson, 1877 (Lepidoptera: Papilionoidea: Papilionidae)

Mitochondrial DNA B Resour. 2024 May 13;9(5):631-635. doi: 10.1080/23802359.2024.2351536. eCollection 2024.

Abstract

In the present study, the complete mitochondrial genome (mitogenome) of the Papilio macilentus (Lepidoptera: Papilionoidea: Papilionidae) was sequenced by next-generation sequencing method. The mitochondrial genome is a circular DNA molecule of 15,264 bp in size with 80.7% AT content, including 37 genes (13 protein-coding genes, 2 rRNA genes, and 22 tRNA genes), and a long non-coding region (Control region). All protein-coding genes are initiated by ATN codons, and terminated with TAA, TAG, or single T. All tRNAs can be folded into common clover leaf secondary structure, except trn-S1. Phylogenetic analyses based on 13 protein-coding genes and 2 rRNA genes using maximum likelihood and Bayesian inference confirmed that P. macilentus and Papilio memnon are clustered into a clade, and revealed the relationships between Papilionini, Troidini, Teinopaippini and Leptocircini.

Keywords: Papilio macilentus; mitogenome; next-generation sequencing; phylogeny.

Grants and funding

This study was supported by the National Science & Technology Fundamental Resources Investigation Program of China [Grant No. 2019FY101800]; the Natural Science Foundation of the Higher Education Institutions of Anhui Province [Grant No. KJ2021A1074]; the Reality Geographical Environment Key Laboratory of Anhui Province [Grant No. 2022PGE014]; and the Natural Science Foundation of the Higher Education Institutions of Anhui Province [Grant No. 2022AH040152].