Recognition and detection of histamine in foods using aptamer modified fluorescence polymer dots sensors

Gan Wu; Zhaoyang Ding; Xilin Dou; Ze Chen; Jing Xie

doi:10.1016/j.saa.2024.124452

Recognition and detection of histamine in foods using aptamer modified fluorescence polymer dots sensors

Spectrochim Acta A Mol Biomol Spectrosc. 2024 Sep 5:317:124452. doi: 10.1016/j.saa.2024.124452. Epub 2024 May 11.

Authors

Gan Wu¹, Zhaoyang Ding², Xilin Dou¹, Ze Chen¹, Jing Xie³

Affiliations

¹ College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.
² College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China; Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation, Shanghai 201306, China; Key Laboratory of Aquatic Products High-quality Utilization, Storage and Transportation (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Shanghai 201306, China; Marine Biomedical Science and Technology Innovation Platform of Lin-gang Special Area, Shanghai 201306, China. Electronic address: zyding@shou.edu.cn.
³ College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China; Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation, Shanghai 201306, China; Key Laboratory of Aquatic Products High-quality Utilization, Storage and Transportation (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Shanghai 201306, China. Electronic address: jxie@shou.edu.cn.

PMID: 38761559
DOI: 10.1016/j.saa.2024.124452

Abstract

Histamine has been known as a momentous cause of biogenic amine poisoning. Therefore, the content of histamine in foods is strictly required to be controlled within a certain range. Here, an aptamer fluorescent sensor was developed for detection of histamine. Poly [(9, 9-di-n-octylfluorenyl-2, 7-diyl)-alt-(benzo [2,1,3] thiadia-zol-4, 8-diyl)] (PF8BT) and the styrene maleic anhydride copolymer (PSMA) were used for the preparation of PF8BT-Polymer dots (PF8BT-Pdots). PF8BT-Pdots and the cyanine3-phosphoramidite (Cy3) were linked through aptamer to achieve the ratiometric detection for histamine. PF8BT-Pdots were partly quenched by Cy3 due to the fluorescence resonance energy transfer (FRET), when the histamine molecule was recognized by aptamer on the surface of PF8BT-Pdots. A linear range (3-21 μmol/L) was obtained for histamine detection with a low limit of detection (LOD = 0.38 μmol/L). PF8BT aptamer Pdots (PF8BT-A) were used to detect histamine in simply treated aquaculture water and tuna. The cell imaging of HeLa cells presented a good biosecurity and outstanding fluorescent imaging capability of PF8BT-A. The aptamer fluorescent sensors provided a new platform for rapid and accurate detection of histamine in aquatic products and had great potential for the application in food safety and quality control.

Keywords: Aptamer; Cell imaging; Food safety; Histamine; Polymer dots.

MeSH terms

Animals
Aptamers, Nucleotide* / chemistry
Biosensing Techniques / methods
Fluorescence Resonance Energy Transfer / methods
Fluorescent Dyes / chemistry
Food Analysis / methods
Food Contamination / analysis
HeLa Cells
Histamine* / analysis
Humans
Limit of Detection
Polymers* / chemistry
Quantum Dots* / chemistry
Spectrometry, Fluorescence / methods

Substances

Histamine
Aptamers, Nucleotide
Polymers
Fluorescent Dyes