Abstract
Expression of Becker-like and full-length human dystrophins was stable for at least 6 months in mdx mouse muscle following intramuscular plasmid DNA injection. Intramuscular injection of a single plasmid DNA encoding both luciferase and dystrophin resulted in stable luciferase expression for at least 2 months in mdx muscle, whereas injection of plasmid DNA encoding only luciferase did not result in stable luciferase expression. These results suggest that expression of either full-length or Becker-like dystrophins protects mdx mouse myofibers from degeneration.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cell Survival
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DNA / administration & dosage
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DNA / genetics
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DNA / metabolism*
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Dystrophin / biosynthesis*
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Dystrophin / metabolism
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Humans
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Injections, Intramuscular
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Luciferases / analysis
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Luciferases / biosynthesis
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Mice
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Mice, Inbred BALB C
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Mice, Inbred C57BL
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Mice, Inbred ICR
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Mice, Neurologic Mutants
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Muscles / metabolism
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Muscles / pathology*
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Muscular Dystrophy, Animal / metabolism
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Muscular Dystrophy, Animal / pathology*
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Muscular Dystrophy, Animal / therapy
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Plasmids / administration & dosage*
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Recombinant Fusion Proteins / biosynthesis
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beta-Galactosidase / analysis
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beta-Galactosidase / biosynthesis
Substances
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Dystrophin
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Recombinant Fusion Proteins
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DNA
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Luciferases
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beta-Galactosidase