Role of epidermal growth factor expression in early mouse embryo lung branching morphogenesis in culture: antisense oligodeoxynucleotide inhibitory strategy

Dev Biol. 1993 Aug;158(2):555-9. doi: 10.1006/dbio.1993.1213.

Abstract

Epidermal growth factor (EGF) expression and branching morphogenesis were inhibited using a 5' 15-mer antisense oligodeoxynucleotide (ODN) directed against EGF precursor mRNA in embryonic mouse lung in culture under chemically defined, serumless conditions. Antisense EGF ODN resulted in > 90% inhibition of EGF immunoreactive peptide synthesis, 75% reduction in branching morphogenesis, 73% decrease in DNA content, 64% decrease in RNA content, 73% decrease in protein synthesis, and 65% decrease in [3H]thymidine incorporation into DNA compared to Embryonic Day 11 controls in culture for 4 days. Sense ODN results were similar to control. Supplementing antisense ODN with EGF partially reversed antisense effects. The results further support a role for EGF in pulmonary organogenesis.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • Epidermal Growth Factor / analysis
  • Epidermal Growth Factor / genetics
  • Epidermal Growth Factor / physiology*
  • Lung / embryology*
  • Lung / metabolism
  • Mice
  • Morphogenesis
  • Oligonucleotides, Antisense / pharmacology*
  • Organ Culture Techniques
  • RNA Precursors / metabolism

Substances

  • Oligonucleotides, Antisense
  • RNA Precursors
  • Epidermal Growth Factor