A comparison of monoclonal antibody immobilization of platelet antigen (MAIPA) and immunobead methods for detection of GPIIb/IIIa antiplatelet antibodies in immune thrombocytopenic purpura

Transfus Med. 1997 Jun;7(2):127-34. doi: 10.1046/j.1365-3148.1997.d01-15.x.

Abstract

In this study we have compared two assays for the detection of autoantibodies GpIIb/IIIa, platelet bound and in serum, in immune thrombocytopenic purpura (ITP). Both assays were found to have a similar sensitivity, but the monoclonal antibody immobilization of platelet antigen (MAIPA) assay was more reproducible than the immunobead assay. The MAIPA and immunobead assay demonstrated an 81% concordance of results for serum antibody detection and a 78% concordance for platelet-associated antibody detection, with an 8-12% incidence of false positive or negative results.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Antibodies, Monoclonal*
  • Antigen-Antibody Reactions / immunology*
  • Antigens, Human Platelet / blood*
  • Autoantibodies / blood*
  • Case-Control Studies
  • Humans
  • Immunosorbent Techniques
  • Middle Aged
  • Platelet Glycoprotein GPIIb-IIIa Complex / immunology*
  • Purpura, Thrombocytopenic, Idiopathic / diagnosis*
  • Purpura, Thrombocytopenic, Idiopathic / immunology
  • Titrimetry

Substances

  • Antibodies, Monoclonal
  • Antigens, Human Platelet
  • Autoantibodies
  • Platelet Glycoprotein GPIIb-IIIa Complex