Abstract
In this study we have compared two assays for the detection of autoantibodies GpIIb/IIIa, platelet bound and in serum, in immune thrombocytopenic purpura (ITP). Both assays were found to have a similar sensitivity, but the monoclonal antibody immobilization of platelet antigen (MAIPA) assay was more reproducible than the immunobead assay. The MAIPA and immunobead assay demonstrated an 81% concordance of results for serum antibody detection and a 78% concordance for platelet-associated antibody detection, with an 8-12% incidence of false positive or negative results.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Aged
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Aged, 80 and over
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Antibodies, Monoclonal*
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Antigen-Antibody Reactions / immunology*
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Antigens, Human Platelet / blood*
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Autoantibodies / blood*
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Case-Control Studies
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Humans
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Immunosorbent Techniques
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Middle Aged
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Platelet Glycoprotein GPIIb-IIIa Complex / immunology*
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Purpura, Thrombocytopenic, Idiopathic / diagnosis*
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Purpura, Thrombocytopenic, Idiopathic / immunology
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Titrimetry
Substances
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Antibodies, Monoclonal
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Antigens, Human Platelet
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Autoantibodies
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Platelet Glycoprotein GPIIb-IIIa Complex