Brasilicardin A, a new terpenoid antibiotic from pathogenic Nocardia brasiliensis: fermentation, isolation and biological activity

H Komaki; A Nemoto; Y Tanaka; H Takagi; K Yazawa; Y Mikami; H Shigemori; J Kobayashi; A Ando; Y Nagata

doi:10.7164/antibiotics.52.13

Brasilicardin A, a new terpenoid antibiotic from pathogenic Nocardia brasiliensis: fermentation, isolation and biological activity

J Antibiot (Tokyo). 1999 Jan;52(1):13-9. doi: 10.7164/antibiotics.52.13.

Authors

H Komaki¹, A Nemoto, Y Tanaka, H Takagi, K Yazawa, Y Mikami, H Shigemori, J Kobayashi, A Ando, Y Nagata

Affiliation

¹ Research Laboratory, Higeta Shoyu Co., Ltd., Choshi, Chiba, Japan.

PMID: 10092191
DOI: 10.7164/antibiotics.52.13

Abstract

A novel tricyclic diterpenoid antibiotic, brasilicardin A, was isolated from the culture broth of Nocardia brasiliensis IFM 0406. The antibiotic exhibited immunosuppressive activity in a mouse mixed lymphocyte reaction (MLR) assay system and its IC50 value was 0.057 microg/ml. Although the inhibitory activity of cyclosporin A (CyA) against IL-2 production was confirmed in the MLR assay system, brasilicardin A did not have the activity. The results of in vitro toxicity testing of brasilicardin A against various human cell lines were compared with those of CyA.

MeSH terms

Aminoglycosides*
Animals
Anti-Bacterial Agents / biosynthesis
Anti-Bacterial Agents / isolation & purification
Anti-Bacterial Agents / pharmacology*
Bacteria / drug effects
Cell Survival / drug effects
Cells, Cultured
Chromatography, High Pressure Liquid
Fermentation
Fungi / drug effects
Humans
Immunophilins / antagonists & inhibitors
Immunosuppressive Agents / isolation & purification
Immunosuppressive Agents / pharmacology*
Interleukin-2 / metabolism
Lymphocyte Culture Test, Mixed
Mice
Microbial Sensitivity Tests
Nocardia / metabolism*

Substances

Aminoglycosides
Anti-Bacterial Agents
Immunosuppressive Agents
Interleukin-2
brasilicardin A
Immunophilins