Development of a surface adhesion immunofluorescent technique for the rapid detection of Salmonella spp. from meat and poultry

J Appl Microbiol. 1999 Apr;86(4):583-90. doi: 10.1046/j.1365-2672.1999.00698.x.

Abstract

A rapid method based on bacterial adhesion was developed for the detection of Salmonella in an enriched meat system. Minced beef samples inoculated with Salm. enteritidis (10 cfu g-1) were incubated overnight (18 h) at 37 degrees C in buffered peptone water. Salmonella enteritidis cells were isolated from the enriched meat sample by surface adhesion onto a polycarbonate membrane attached to a glass slide. The organisms attached to this polycarbonate membrane were subsequently visualized using immunofluorescent microscopy. The technique had a detection level of log10 3.5 Salmonella ml-1. The surface adhesion immunofluorescent technique correlated well with Salmonella plate counts (r2 = 0.99). Application of the rapid method to retail beef and poultry samples (n = 100) confirmed the correlation between this technique and traditional microbiological procedures. Thirty-one retail samples were reported positive for Salmonella species. No false positives or negatives were recorded for the rapid method.

MeSH terms

  • Animals
  • Bacterial Adhesion*
  • Bacteriological Techniques
  • Cattle
  • Colony Count, Microbial
  • Culture Media
  • Fluorescent Antibody Technique*
  • Meat / microbiology*
  • Membranes, Artificial
  • Poultry / microbiology*
  • Salmonella / growth & development
  • Salmonella / isolation & purification*
  • Salmonella / physiology
  • Serotyping

Substances

  • Culture Media
  • Membranes, Artificial