Isotype-specific changes in the amount of beta-tubulin RNA in synchronized tobacco BY2 cells

Cell Struct Funct. 1999 Jun;24(3):117-22. doi: 10.1247/csf.24.117.

Abstract

The 3'-ends of the beta-tubulin cDNA were amplified from tobacco BY2 polyA+ RNA. According to the differences in the predicted amino acid sequence at the extreme C-terminal, they were grouped into three different isotypes, NTB1 in which "EEGDYYEEDEEDLNEA", NTB2 in which "EEEYYEDEEEA QED" and NTB3 in which "DECEYEEEEEYDHEGN" follows the conservative "YQQYQDATAD" sequence. Using unique 3'-untranslated regions as probes, changes in the RNA levels of each beta-tubulin isotype were determined by dot-blot hybridization. The levels exhibited characteristic rhythms in the cell cycle. NTB1 RNA was highest in S phase in comparison to NTB2 RNA level which was highest in late G2. On the other hand, NTB3 RNA level was highest in early G2.

MeSH terms

  • 3' Untranslated Regions / genetics
  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Northern
  • Cell Cycle / physiology
  • Cell Line / metabolism
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • Gene Library
  • Immunoblotting
  • Models, Genetic
  • Molecular Sequence Data
  • Nicotiana / genetics*
  • Nicotiana / metabolism
  • Plants, Toxic*
  • Polymerase Chain Reaction
  • Protein Isoforms
  • RNA / analysis*
  • RNA, Messenger / genetics
  • Tubulin / genetics*
  • Tubulin / metabolism

Substances

  • 3' Untranslated Regions
  • DNA, Complementary
  • Protein Isoforms
  • RNA, Messenger
  • Tubulin
  • RNA

Associated data

  • GENBANK/U91562
  • GENBANK/U91563
  • GENBANK/U91564