Abstract
Bacterial lipopolysaccharide (LPS) stably induced the protein kinase C substrate, MacMARCKS, in murine resident peritoneal macrophages; initial induction of MacMARCKS mRNA was detected within 15 min and was protein synthesis-independent. This response was observed in the macrophage cell line RAW264, and occurred also in response to plasmid DNA, a partial mimetic of other responses to LPS. In murine bone marrow-derived macrophages, MacMARCKS was expressed constitutively due to induction by macrophage colony-stimulating factor. Nuclear run-on transcription revealed that, like tumor necrosis factor alpha (TNF-alpha), MacMARCKS was transcribed constitutively in RAW264 cells. The MacMARCKS promoter was sequenced to -1.7 kb and the transcription start site determined. Transient transfections of RAW264 cells revealed that the 113-bp GC-rich proximal promoter contained all the elements required for both high basal activity and 15- to 20-fold activation by LPS.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Base Sequence
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Bone Marrow Cells / drug effects
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Bone Marrow Cells / metabolism
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Calmodulin-Binding Proteins
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Gene Expression Regulation / drug effects*
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Gene Expression Regulation, Neoplastic / drug effects
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Intracellular Signaling Peptides and Proteins
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Lipopolysaccharides / pharmacology*
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Macrophages / drug effects*
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Macrophages / metabolism
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Macrophages, Peritoneal / drug effects
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Macrophages, Peritoneal / metabolism
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Membrane Proteins / biosynthesis
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Membrane Proteins / genetics*
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Mice
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Microfilament Proteins
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Molecular Sequence Data
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Neoplasm Proteins / biosynthesis
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Organ Specificity
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Promoter Regions, Genetic
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Protein Kinase C / metabolism
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RNA, Messenger / biosynthesis
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RNA, Neoplasm / biosynthesis
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Recombinant Fusion Proteins / biosynthesis
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Recombinant Fusion Proteins / genetics
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Transcription, Genetic / drug effects
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Transfection
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Tumor Cells, Cultured / drug effects
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Tumor Cells, Cultured / metabolism
Substances
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Calmodulin-Binding Proteins
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Intracellular Signaling Peptides and Proteins
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Lipopolysaccharides
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Marcksl1 protein, mouse
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Membrane Proteins
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Microfilament Proteins
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Neoplasm Proteins
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RNA, Messenger
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RNA, Neoplasm
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Recombinant Fusion Proteins
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Protein Kinase C