A simple and rapid method for the preparation of a cell-free extract with CCAAT-binding activity from filamentous fungi

M Kato; K Hayashi; T Kobayashi; N Tsukagoshi

doi:10.1271/bbb.64.455

A simple and rapid method for the preparation of a cell-free extract with CCAAT-binding activity from filamentous fungi

Biosci Biotechnol Biochem. 2000 Feb;64(2):455-7. doi: 10.1271/bbb.64.455.

Authors

M Kato¹, K Hayashi, T Kobayashi, N Tsukagoshi

Affiliation

¹ Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University, Japan. kato@agr.nagoya-u.ac.jp

PMID: 10737212
DOI: 10.1271/bbb.64.455

Abstract

A simple and rapid method for the preparation of a cell-free extract with the CCAAT-binding activity was established with Aspergillus nidulans as a model fungus. Proteins were extracted with 6 M guanidine hydrochloride directly from mycelia and renatured by dialysis. This method was found applicable to other filamentous fungi such as Aspergillus oryzae and Trichoderma viride.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aspergillus / metabolism*
CCAAT-Enhancer-Binding Proteins
Cell-Free System
DNA-Binding Proteins / metabolism*
Electrophoresis
Fungal Proteins / metabolism*
Nuclear Proteins / metabolism*
Protein Binding
Recombinant Proteins / metabolism
Trichoderma / metabolism*

Substances

CCAAT-Enhancer-Binding Proteins
DNA-Binding Proteins
Fungal Proteins
Nuclear Proteins
Recombinant Proteins