Cloning and sequence of the gene encoding a novel cefotaxime-hydrolyzing beta-lactamase (CTX-M-9) from Escherichia coli in Spain

Antimicrob Agents Chemother. 2000 Jul;44(7):1970-3. doi: 10.1128/AAC.44.7.1970-1973.2000.

Abstract

A new CTX-M-type beta-lactamase (CTX-M-9) has been cloned from a clinical cefotaxime-resistant Escherichia coli strain. Despite the close identity that exists between the CTX-M-9 and Toho-2 beta-lactamases (88%), the 35 amino acids located between residues Ala-185 and Ala-219 are totally different in both enzymes. Outside of this region there are only six amino acids substitutions between both proteins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Cefotaxime / metabolism
  • Cefotaxime / pharmacology
  • Cephalosporins / metabolism
  • Cephalosporins / pharmacology
  • Cloning, Molecular
  • Escherichia coli / enzymology
  • Escherichia coli / genetics*
  • Escherichia coli Proteins*
  • Hydrolysis
  • Molecular Sequence Data
  • Sequence Homology, Amino Acid
  • Spain
  • beta-Lactamases / genetics*
  • beta-Lactamases / metabolism

Substances

  • Cephalosporins
  • Escherichia coli Proteins
  • CTX-M-9 protein, E coli
  • beta-Lactamases
  • Cefotaxime