Replication and expression of constructed plasmid chimeras in transformed Escherichia coli--a review

Basic Life Sci. 1975:5A:335-44. doi: 10.1007/978-1-4684-2895-7_46.

Abstract

EcoRI restriction-endonuclease-generated fragments of bacterial plasmids isolated from Staphylococcus aureus or Escherichia coli, or of amplified DNA coding for the 18S and 28S ribosomal RNA of Xenopus laevis, have been linked to the pSC101 plasmid replicon and introduced into E. coli by transformation. The constructed plasmid chimeras can be cloned as stable replicons in E. coli, where they synthesize RNA and/or protein products specified by their component genes.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Animals
  • Anura
  • Base Sequence
  • DNA Replication*
  • DNA Restriction Enzymes / metabolism
  • Escherichia coli / enzymology
  • Escherichia coli / metabolism*
  • Extrachromosomal Inheritance*
  • Genetic Code
  • Nucleic Acid Conformation
  • Plasmids*
  • RNA, Ribosomal / biosynthesis
  • Species Specificity
  • Staphylococcus / metabolism
  • Transcription, Genetic
  • Transformation, Genetic*
  • Xenopus

Substances

  • RNA, Ribosomal
  • DNA Restriction Enzymes