Genomic map of Clostridium perfringens strain 13

Microbiol Immunol. 2001;45(2):179-89. doi: 10.1111/j.1348-0421.2001.tb01278.x.

Abstract

A physical and genetic map of Clostridium perfringens strain 13 was constructed. C. perfringens strain 13 was found to have a 3.1-Mb chromosome and a large 50-kb plasmid, indicating that strain 13 has a relatively small genome among C. perfringens strains. A total of 313 genetic markers were mapped on the chromosome of strain 13. Compared with the physical and genetic map of C. perfringens CPN50, strain 13 had a quite similar genome organization, but with a large deletion (approximately 400 kb) in a particular segment of the chromosome. Among several toxin genes, a beta2 toxin gene that is a novel virulence gene in C. perfringens was found to be located on the 50-kb plasmid.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Toxins / genetics
  • Blotting, Southern
  • Chromosome Deletion
  • Chromosomes, Bacterial / genetics*
  • Cloning, Molecular
  • Clostridium perfringens / genetics*
  • Clostridium perfringens / pathogenicity
  • DNA Probes
  • Electrophoresis, Gel, Pulsed-Field
  • Genes, Bacterial / genetics*
  • Genome, Bacterial
  • Physical Chromosome Mapping*
  • Plasmids / genetics
  • Sequence Analysis, DNA
  • Sequence Homology, Nucleic Acid
  • Virulence / genetics

Substances

  • Bacterial Toxins
  • DNA Probes