A phosphodiesterase from ascites carcinoma Krebs II cells specifically cleaves the bond between VPg and RNA of encephalomyocarditis virus

A Y Gulevich; R A Yusupova; Y F Drygin

doi:10.1023/a:1010220401336

A phosphodiesterase from ascites carcinoma Krebs II cells specifically cleaves the bond between VPg and RNA of encephalomyocarditis virus

Biochemistry (Mosc). 2001 Mar;66(3):345-9. doi: 10.1023/a:1010220401336.

Authors

A Y Gulevich¹, R A Yusupova, Y F Drygin

Affiliation

¹ Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, 119899, Russia.

PMID: 11333162
DOI: 10.1023/a:1010220401336

Abstract

The substrate specificity of the "unlinking" enzyme from ascite carcinoma Krebs II cells has been investigated. The enzyme specifically splits the interpolymeric phosphodiester bond between Kp and the 5;-terminal phosphate group of the uridylic acid residue in the Kp-pUpUpGp complex.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Ascites / enzymology*
Carcinoma / enzymology*
Chromatography, Thin Layer
Encephalomyocarditis virus / metabolism*
Hydrolysis
Mice
Phosphoric Diester Hydrolases / chemistry*
Protein Binding
RNA / metabolism*
Tumor Cells, Cultured
Viral Core Proteins / metabolism*

Substances

Viral Core Proteins
RNA
Phosphoric Diester Hydrolases