Sequence and gene expression analyses of plasmid pHPM8 from Helicobacter pylori reveal the presence of two operons with putative roles in plasmid replication and antibiotic activity

Plasmid. 2001 Nov;46(3):223-8. doi: 10.1006/plas.2001.1541.

Abstract

The DNA sequence of a 7.8-kb Helicobacter pylori plasmid, pHPM8, was determined. Six open reading frames (ORFs) were present. Ribonuclease protection studies showed that ORF1/ORF2 and ORF3/ORF4 genes are organized in operons possibly involved in plasmid replication and in production of a peptide with antibiotic activity, respectively. Finding areas of pHPM8 with a high level of identity to H. pylori chromosomal DNA supported the hypothesis that recombination occurs between plasmids and the chromosome of H. pylori.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / genetics
  • Chromosomes, Bacterial / genetics
  • DNA Replication / genetics*
  • DNA, Bacterial / biosynthesis
  • DNA, Bacterial / genetics*
  • Drug Resistance / genetics*
  • Gene Expression Regulation, Bacterial*
  • Genes, Bacterial*
  • Genes, Overlapping
  • Helicobacter pylori / drug effects
  • Helicobacter pylori / genetics*
  • Molecular Sequence Data
  • Open Reading Frames
  • Operon / genetics*
  • Plasmids / genetics*
  • Sequence Alignment
  • Sequence Homology, Amino Acid

Substances

  • Bacterial Proteins
  • DNA, Bacterial

Associated data

  • GENBANK/AF275307