Abstract
The DNA sequence of a 7.8-kb Helicobacter pylori plasmid, pHPM8, was determined. Six open reading frames (ORFs) were present. Ribonuclease protection studies showed that ORF1/ORF2 and ORF3/ORF4 genes are organized in operons possibly involved in plasmid replication and in production of a peptide with antibiotic activity, respectively. Finding areas of pHPM8 with a high level of identity to H. pylori chromosomal DNA supported the hypothesis that recombination occurs between plasmids and the chromosome of H. pylori.
Copyright 2001 Elsevier Science.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Bacterial Proteins / genetics
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Chromosomes, Bacterial / genetics
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DNA Replication / genetics*
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DNA, Bacterial / biosynthesis
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DNA, Bacterial / genetics*
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Drug Resistance / genetics*
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Gene Expression Regulation, Bacterial*
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Genes, Bacterial*
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Genes, Overlapping
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Helicobacter pylori / drug effects
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Helicobacter pylori / genetics*
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Molecular Sequence Data
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Open Reading Frames
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Operon / genetics*
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Plasmids / genetics*
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Sequence Alignment
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Sequence Homology, Amino Acid
Substances
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Bacterial Proteins
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DNA, Bacterial