Objective: To explore the role and the significance of TNFalpha and NO in the early renal damage in burned rats complicated with endotoxemia.
Methods: The rats inflicted with 20% TBSA III degree burn and injected intraperitoneally with endotoxin (1 mg/kg) were employed as the model of MOD. The renal pathomorphology was observed at 0.5, 1, 3, 6, 12, 24, 48, 72 postburn hours. Concomitantly, the changes of renal and serum TNFalpha and NO were investigated and in situ hybridization for TNFalpha mRNA and immunohistochemical staining for iNOS were performed.
Result: The expressions of renal iNOS and TNFalpha mRNA, the NO synthesis and the serum levels of NO and TNFalpha in BCEG (burns complicated with endotoxin group) were obviously higher and appeared earlier than those in SBG (simple burns group) and SEG(simple endotoxin group).
Conclusion: The renal TNFalpha and NO increased significantly in rats subjected to burn complicated with endotoxemia, which might be important factors causing renal damage and the alterations in renal hemodynamics.