Lymphocyte subsets in inline filtered packed red blood cell units: comparison between low and high spin procedures

Transfus Apher Sci. 2002 Jun;26(3):167-74. doi: 10.1016/s1473-0502(02)00009-5.

Abstract

Lymphocyte subsets were determined in 20 packed red blood cell units (PRC) before and after filtration (FPRC) with the Pall Leukotrap RC inline filter system; 10 units were prepared by low spin and platelet rich plasma (PRP) removal (Group A) and 10 with high spin, plasma and buffy-coat (BC) removal (Group B). Flow cytometry was employed for white blood cell (WBC) enumeration and phenotype analysis. Median WBCs in prefiltered units was 2.08 x 10(9) (Group A) vs. 0.8 x 10(9) (Group B) (p < 0.0001). Five Group A and three Group B filtered units had WBC counts above the limit of detection (LD), median values being 25.59 and 3.08 x 10(3), respectively. Whereas CD3+, CD3+CD4+ and CD3+CD8+ lymphocyte subsets were assessable in 20-40% of Group A units, inline filtration of Group B units lowered lymphocytes below the LD of the present study. Post-filtration CD19+ lymphocytes were below the LD in all the 20 units.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Validation Study

MeSH terms

  • Antigens, CD / analysis
  • Blood Specimen Collection
  • Cell Separation / methods*
  • Cell Separation / standards*
  • Centrifugation / methods
  • Erythrocyte Transfusion / standards
  • Erythrocytes / cytology*
  • Filtration / methods
  • Flow Cytometry
  • Humans
  • Immunophenotyping
  • Leukocyte Count / instrumentation
  • Leukocyte Count / methods
  • Lymphocyte Subsets / cytology*

Substances

  • Antigens, CD