Cell dynamics during somite boundary formation revealed by time-lapse analysis

Science. 2002 Nov 1;298(5595):991-5. doi: 10.1126/science.1075544.

Abstract

We follow somite segmentation in living chick embryos and find that the shaping process is not a simple periodic slicing of tissue blocks but a much more carefully choreographed separation in which the somite pulls apart from the segmental plate. Cells move across the presumptive somite boundary and violate gene expression boundaries thought to correlate with the site of the somite boundary. Similarly, cells do not appear to be preassigned to a given somite as they leave the node. The results offer a detailed picture of somite shaping and provide a spatiotemporal framework for linking gene expression with cell movements.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Boron Compounds
  • Carbocyanines
  • Cell Adhesion
  • Cell Movement
  • Cell Size
  • Central Nervous System / embryology
  • Ceramides
  • Chick Embryo
  • Epithelial Cells / cytology
  • Gene Expression Profiling
  • Gene Expression*
  • Mesoderm / cytology*
  • Mesoderm / physiology
  • Microscopy, Confocal
  • Models, Biological
  • Receptor, EphA4 / genetics
  • Somites / cytology*
  • Somites / physiology*
  • Time Factors

Substances

  • 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene
  • Boron Compounds
  • Carbocyanines
  • Ceramides
  • 3,3'-dioctadecylindocarbocyanine
  • Receptor, EphA4