Identification of Histoplasma capsulatum from culture extracts by real-time PCR

Jose Martagon-Villamil; Nabin Shrestha; Mary Sholtis; Carlos M Isada; Gerri S Hall; Terry Bryne; Barbara A Lodge; L Barth Reller; Gary W Procop

doi:10.1128/JCM.41.3.1295-1298.2003

Identification of Histoplasma capsulatum from culture extracts by real-time PCR

J Clin Microbiol. 2003 Mar;41(3):1295-8. doi: 10.1128/JCM.41.3.1295-1298.2003.

Authors

Jose Martagon-Villamil¹, Nabin Shrestha, Mary Sholtis, Carlos M Isada, Gerri S Hall, Terry Bryne, Barbara A Lodge, L Barth Reller, Gary W Procop

Affiliation

¹ Division of Infectious Diseases. Section of Clinical Microbiology, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA.

Abstract

We designed and tested a real-time LightCycler PCR assay for Histoplasma capsulatum that correctly identified the 34 H. capsulatum isolates in a battery of 107 fungal isolates tested and also detected H. capsulatum in clinical specimens from three patients that were culture positive for this organism.

MeSH terms

DNA, Fungal / analysis
Histoplasma / genetics
Histoplasma / isolation & purification*
Humans
Microbiological Techniques
Polymerase Chain Reaction / methods*

Substances

DNA, Fungal