Hippocampal kindling increases the expression of glutamate receptor-A Flip and -B Flip mRNA in dentate granule cells

Neurosci Lett. 1992 Dec 14;148(1-2):51-4. doi: 10.1016/0304-3940(92)90802-e.

Abstract

The level of the mRNAs encoding the AMPA-selective glutamate receptors-A and -B, alternatively splice variants, Flip and Flop, was studied by in situ hybridization in the brains of rats kindled by Schaffer collateral/commissural-fiber stimulation. In comparison to control animals, the expression level of the Flip variant of both GluR-A and GluR-B mRNAs was bilaterally enhanced in the dentate granule neurons of kindled animals 24 h after last-generalized seizure, whereas no obvious alterations were observed in the GluR-A Flop and GluR-B Flop mRNA variants. In kindled animals, studied 1 month after the last seizure, GluR-A Flip and GluR-B Flip mRNA had returned to control levels. We suggest that these changes may result in an enhanced glutamate receptor sensitivity in the fascia dentata during kindling.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alternative Splicing*
  • Animals
  • Electric Stimulation
  • Evoked Potentials
  • Genetic Variation
  • Hippocampus / cytology
  • Hippocampus / physiology*
  • Hippocampus / physiopathology
  • Kindling, Neurologic*
  • Male
  • Pyramidal Tracts / cytology
  • Pyramidal Tracts / physiology
  • RNA, Messenger / metabolism*
  • Rats
  • Rats, Wistar
  • Receptors, Glutamate / biosynthesis
  • Receptors, Glutamate / genetics*
  • Seizures / physiopathology
  • Time Factors

Substances

  • RNA, Messenger
  • Receptors, Glutamate