Abstract
We report the cloning of a human gene encoding the 5-hydroxytryptamine1B receptor. The receptor has the characteristics of a G-protein-linked receptor and is most homologous to the human 5-HT1D receptor. This human 5-HT receptor gene, most abundantly expressed in striatum, is localized on chromosome 6, at 6q13, and the gene encoding the 5-HT1D receptor is localized on chromosome 1. Radioligand studies indicate that the affinity of [3H]5-HT is 16 +/- 2 nM. Drug competition studies indicate that the receptor displays high affinity (i.e. less than 40 nM) for 5-HT, 5-carboxyamidotryptamine, methiothepin, and metergoline.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Autoradiography
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Base Sequence
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Binding, Competitive
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Blotting, Northern
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Blotting, Southern
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Brain / metabolism*
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Chromosomes, Human, Pair 6*
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Cloning, Molecular
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DNA / genetics
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DNA / isolation & purification
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Genomic Library
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Humans
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Molecular Sequence Data
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Oligodeoxyribonucleotides
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Organ Specificity
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Pituitary Gland / metabolism*
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Polymerase Chain Reaction / methods
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Polymorphism, Restriction Fragment Length*
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RNA, Messenger / genetics
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RNA, Messenger / isolation & purification
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Rats
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Receptors, Serotonin / analysis
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Receptors, Serotonin / genetics*
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Receptors, Serotonin / metabolism*
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Recombinant Proteins / metabolism
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Restriction Mapping
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Serotonin / metabolism
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Tritium
Substances
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Oligodeoxyribonucleotides
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RNA, Messenger
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Receptors, Serotonin
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Recombinant Proteins
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Tritium
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Serotonin
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DNA
Associated data
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GENBANK/L01674
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GENBANK/L01675
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GENBANK/L01676
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GENBANK/L01677
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GENBANK/L17004
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GENBANK/M83326
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GENBANK/M83327
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GENBANK/M83328
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GENBANK/M83368
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GENBANK/M84986