Abstract
The mammalian splicing factor SC35 is required for the first step in the splicing reaction and for spliceosome assembly. The cloning and characterization of a complementary DNA encoding this protein revealed that it is a member of a family of splicing factors that includes mammalian SF2/ASF. This family of proteins is characterized by the presence of a ribonucleoprotein (RNP)-type RNA binding motif and a carboxyl-terminal serine-arginine-rich (SR) domain. A search of the DNA sequence database revealed that the thymus-specific exon (ET) of the c-myb proto-oncogene is encoded on the antisense strand of the SC35 gene.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Baculoviridae / genetics
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Base Sequence
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Binding Sites
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Blotting, Northern
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Cell Line
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Cloning, Molecular
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Codon
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DNA / chemistry
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DNA / isolation & purification*
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Exons
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Humans
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Molecular Sequence Data
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Nuclear Proteins*
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Proteins / chemistry
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Proteins / genetics*
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Proto-Oncogene Mas
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Proto-Oncogene Proteins / genetics
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Proto-Oncogene Proteins c-myb
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RNA / metabolism
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RNA Splicing*
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Ribonucleoproteins*
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Sequence Homology, Nucleic Acid
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Serine-Arginine Splicing Factors
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Transfection
Substances
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Codon
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MAS1 protein, human
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Nuclear Proteins
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Proteins
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Proto-Oncogene Mas
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Proto-Oncogene Proteins
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Proto-Oncogene Proteins c-myb
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Ribonucleoproteins
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SRSF2 protein, human
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Serine-Arginine Splicing Factors
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RNA
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DNA