Isolation of a complementary DNA that encodes the mammalian splicing factor SC35

Science. 1992 Apr 24;256(5056):535-8. doi: 10.1126/science.1373910.

Abstract

The mammalian splicing factor SC35 is required for the first step in the splicing reaction and for spliceosome assembly. The cloning and characterization of a complementary DNA encoding this protein revealed that it is a member of a family of splicing factors that includes mammalian SF2/ASF. This family of proteins is characterized by the presence of a ribonucleoprotein (RNP)-type RNA binding motif and a carboxyl-terminal serine-arginine-rich (SR) domain. A search of the DNA sequence database revealed that the thymus-specific exon (ET) of the c-myb proto-oncogene is encoded on the antisense strand of the SC35 gene.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Baculoviridae / genetics
  • Base Sequence
  • Binding Sites
  • Blotting, Northern
  • Cell Line
  • Cloning, Molecular
  • Codon
  • DNA / chemistry
  • DNA / isolation & purification*
  • Exons
  • Humans
  • Molecular Sequence Data
  • Nuclear Proteins*
  • Proteins / chemistry
  • Proteins / genetics*
  • Proto-Oncogene Mas
  • Proto-Oncogene Proteins / genetics
  • Proto-Oncogene Proteins c-myb
  • RNA / metabolism
  • RNA Splicing*
  • Ribonucleoproteins*
  • Sequence Homology, Nucleic Acid
  • Serine-Arginine Splicing Factors
  • Transfection

Substances

  • Codon
  • MAS1 protein, human
  • Nuclear Proteins
  • Proteins
  • Proto-Oncogene Mas
  • Proto-Oncogene Proteins
  • Proto-Oncogene Proteins c-myb
  • Ribonucleoproteins
  • SRSF2 protein, human
  • Serine-Arginine Splicing Factors
  • RNA
  • DNA

Associated data

  • GENBANK/M90104