Heteronuclear NMR of DNA with the heteronucleus in natural abundance: facilitated assignment and extraction of coupling constants

P Schmieder; J H Ippel; H van den Elst; G A van der Marel; J H van Boom; C Altona; H Kessler

doi:10.1093/nar/20.18.4747

Heteronuclear NMR of DNA with the heteronucleus in natural abundance: facilitated assignment and extraction of coupling constants

Nucleic Acids Res. 1992 Sep 25;20(18):4747-51. doi: 10.1093/nar/20.18.4747.

Authors

P Schmieder¹, J H Ippel, H van den Elst, G A van der Marel, J H van Boom, C Altona, H Kessler

Affiliation

¹ Organisch-Chemisches Institut, TU München, Garching, Germany.

Abstract

Two heteronuclear proton-carbon NMR experiments are applied to the DNA-octamer d(TTGGCCAA)2 with carbon in natural abundance. They lead to a complete assignment of the carbon resonances of the sugars and bases. In addition, several heteronuclear coupling constants, proton-carbon as well as proton-phosphorous and phosphorous-carbon, were determined. The information can be obtained in a reasonable measuring time and offers valuable information for a detailed picture of DNA structure.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
DNA / chemistry*
Magnetic Resonance Spectroscopy / methods
Nucleic Acid Conformation*
Oligodeoxyribonucleotides / chemistry*

Substances

Oligodeoxyribonucleotides
DNA