Degranulation and superoxide production depend on cholesterol in PLB-985 cells

Biochem Biophys Res Commun. 2003 Oct 31;310(4):1241-6. doi: 10.1016/j.bbrc.2003.09.150.

Abstract

The effect of agents disrupting cholesterol-rich microdomains of the cell membrane was studied on the chemoattractant receptor (FPR and FRPL1) coupled effector responses of promyelocytic PLB-985 cells. Both methyl-beta-cyclodextrin (MbetaCD) and filipin III inhibited exocytosis of primary granules and O(2)(.-) production induced by stimulation of either chemotactic receptor. Alteration of calcium homeostasis of MbetaCD-treated cells does not account for the impairment of the effector responses. Disruption of microfilaments by cytochalasin B (CB) partially reverses the inhibitory effect of cholesterol depletion. Our results provide functional support for the involvement of cholesterol-rich membrane domains in the signaling of chemotactic receptors and call the attention to the possible role of microfilaments in the organization of lipid microdomains.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Cell Degranulation* / drug effects
  • Cell Line
  • Cholesterol / metabolism*
  • Cyclodextrins / pharmacology
  • Filipin / pharmacology
  • Superoxides / metabolism*
  • beta-Cyclodextrins*

Substances

  • Cyclodextrins
  • beta-Cyclodextrins
  • methyl-beta-cyclodextrin
  • Superoxides
  • Filipin
  • Cholesterol