[Recombinant OspC identification and antigenicity detection from Borrelia burgdorferi PD91 in China]

Zhonghua Liu Xing Bing Xue Za Zhi. 2003 Oct;24(10):917-9.
[Article in Chinese]

Abstract

Objective: To recombine OspC gene from Borrelia burgdorferi PD91 of China and expressed it in E. coli for early diagnosis of Lyme disease.

Methods: The OspC gene was amplified from the genome of Borrelia burgdorferi PD91 strain by polymerase chain reaction and recombined with plasmid PET-11D. The recombinant plasmid PET-11D-OspC was identified with PCR, restriction endonuclease analysis and sequencing. The antigenicity was verified with Western Blot.

Results: OspC gene was cloned correctly into vector PET-11D. The resultant sequence was definitely different from the published sequence. The recombinant OspC seemed to have had strong antigenicity.

Conclusion: The findings laid basis for the studies on early diagnosis of Lyme disease.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, Bacterial*
  • Bacterial Outer Membrane Proteins / genetics*
  • Bacterial Outer Membrane Proteins / immunology
  • Blotting, Western
  • Borrelia burgdorferi Group / immunology*
  • Escherichia coli / genetics
  • Humans
  • Lyme Disease / diagnosis
  • Polymerase Chain Reaction
  • Recombinant Proteins / analysis
  • Recombinant Proteins / immunology

Substances

  • Antigens, Bacterial
  • Bacterial Outer Membrane Proteins
  • OspC protein
  • Recombinant Proteins