In recent years, biomechanics researchers have increasingly used dynamic magnetic resonance imaging techniques, such as cine phase contrast (cine PC), to study muscle and bone motion in vivo. Magnetic resonance imaging provides a non-invasive tool to visualize the anatomy and measure musculoskeletal tissue velocities during joint motion. Current application of cine PC magnetic resonance imaging in biomechanics includes study of knee joint kinematics, tendon strain, and skeletal muscle displacement and shortening. This paper article reviews the use of cine PC magnetic resonance imaging for quantification of skeletal muscle motion. The imaging studies presented examine the relative motion of the knee flexor and extensor muscles after orthopedic surgery and examine the uniformity of shortening within the biceps brachii muscle. The current challenges and limitations of using cine PC magnetic resonance imaging in biomechanics research are addressed as well as opportunities for future studies of skeletal muscle motion using dynamic magnetic resonance imaging.