Performance of a commercial, type-specific enzyme-linked immunosorbent assay for detection of herpes simplex virus type 2-specific antibodies in Ugandans

J Clin Microbiol. 2004 Apr;42(4):1794-6. doi: 10.1128/JCM.42.4.1794-1796.2004.

Abstract

Two hundred forty-eight human immunodeficiency virus (HIV)-positive and 496 HIV-negative subjects in Uganda were tested by HerpeSelect herpes simplex virus type 2 enzyme-linked immunosorbent assay (ELISA) and Western blotting to optimize the ELISA for use in this population. A higher index cutoff value was required for optimal sensitivity and specificity, and overall performance of the assay was not affected by HIV status.

Publication types

  • Evaluation Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antibodies, Viral / blood*
  • Blotting, Western
  • Enzyme-Linked Immunosorbent Assay
  • HIV Seronegativity
  • HIV Seropositivity / complications
  • Herpes Genitalis / diagnosis*
  • Herpesvirus 2, Human / immunology*
  • Humans
  • Reagent Kits, Diagnostic*
  • Sensitivity and Specificity
  • Uganda

Substances

  • Antibodies, Viral
  • Reagent Kits, Diagnostic