Abstract
A plasmid containing human coagulation factor VII (hFVII) complementary DNA regulated by a cytomegalovirus promoter was microinjected into fertilized eggs of zebrafish, African catfish, and tilapia. The active form of hFVll was detected in the fish embryos by various assays. This positive expression of human therapeutic protein in fish embryos demonstrates the possibility of exploitation of transgenic fish as bioreactors.
MeSH terms
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Animals
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Bioreactors*
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Blood Coagulation / drug effects
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Cytomegalovirus / genetics
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DNA, Complementary / genetics
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Enzyme-Linked Immunosorbent Assay
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Factor VII / genetics
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Factor VII / metabolism*
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Factor VII / pharmacology
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Fishes / metabolism*
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Genetic Vectors / genetics
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Humans
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Microinjections
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Photometry
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Promoter Regions, Genetic / genetics
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Transgenes / genetics*
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Zygote / metabolism*
Substances
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DNA, Complementary
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Factor VII