Fluoroquinolone resistance in clinical isolates of Streptococcus pneumoniae from Asian countries: ANSORP study

Microb Drug Resist. 2004 Spring;10(1):37-42. doi: 10.1089/107662904323047781.

Abstract

Seventeen clinical isolates of Streptococcus pneumoniae showing reduced susceptibility to ciprofloxacin (MIC >/= 4 micro g/ml) collected from eight different Asian countries were analyzed by antimicrobial susceptibility, serotyping, pulsed-field gel electrophoresis (PFGE), and DNA sequencing of the quinolone resistance-determining regions (QRDRs) in gyrA, gyrB, parC, and parE. All isolates but one showed more than one amino acid alteration in QRDRs of four responsible genes. Ile460 --> Val in parE was the most common mutation. Data suggest that Lys137 --> Asn in parC may be a primary step in the development of high-level and multiple FQ resistance. An additional mutation of Ser81 --> Phe in gyrA resulted in high-level resistance to ciprofloxacin, levofloxacin, and gatifloxacin, whereas Ser79 --> Phe in parC may exert an important role in the development of moxifloxacin resistance. Two novel amino acid changes in gyrB, Ala390 --> Val and Asn423 --> Thr, were found. Data from PFGE suggest an introduction and local spread of multiple resistant Spain(23F)-1 clone in Hong Kong, but isolates from other Asian countries were not related to this clone.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / pharmacology*
  • Asia / epidemiology
  • Ciprofloxacin / pharmacology
  • DNA Gyrase / genetics
  • DNA Topoisomerase IV / genetics
  • DNA, Bacterial / genetics
  • Drug Resistance, Bacterial
  • Electrophoresis, Gel, Pulsed-Field
  • Fluoroquinolones / pharmacology*
  • Microbial Sensitivity Tests
  • Pneumococcal Infections / epidemiology*
  • Pneumococcal Infections / microbiology*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Serotyping
  • Streptococcus pneumoniae / drug effects*

Substances

  • Anti-Bacterial Agents
  • DNA, Bacterial
  • Fluoroquinolones
  • Ciprofloxacin
  • DNA Topoisomerase IV
  • DNA Gyrase