A Pinus radiata AAA-ATPase, the expression of which increases with tree ageing

L Borja Diego; María Berdasco; Mario F Fraga; M Jesús Cañal; Roberto Rodríguez; Carmen Castresana

doi:10.1093/jxb/erh171

A Pinus radiata AAA-ATPase, the expression of which increases with tree ageing

J Exp Bot. 2004 Jul;55(402):1597-9. doi: 10.1093/jxb/erh171. Epub 2004 Jun 18.

Authors

L Borja Diego¹, María Berdasco, Mario F Fraga, M Jesús Cañal, Roberto Rodríguez, Carmen Castresana

Affiliation

¹ Laboratorio Fisiología Vegetal, Departamento BOS, Facultad de Biología, Universidad de Oviedo, C/ Catedrático Rodrigo Uría s/n. E-33071, Oviedo, Spain.

PMID: 15208342
DOI: 10.1093/jxb/erh171

Abstract

Age-dependent changes in gene expression profiles were studied in vegetative Pinus radiata buds by means of differential display. Among several candidate cDNAs, a 327 bp fragment that shows high homology with an Arabidopsis thaliana 20S proteasome ATPase designated RPT5a was found. Northern hybridization confirmed that the accumulation of this transcript increases with tree ageing, suggesting a possible role of this AAA-ATPase gene in development-related specific proteolysis.

MeSH terms

Adenosine Triphosphatases / genetics*
Amino Acid Sequence
Conserved Sequence
Cysteine Endopeptidases / genetics*
Gene Expression Regulation, Developmental / genetics
Gene Expression Regulation, Enzymologic / genetics
Gene Expression Regulation, Plant / genetics*
Molecular Sequence Data
Multienzyme Complexes / genetics*
Pinus / enzymology
Pinus / genetics*
Pinus / growth & development*
Proteasome Endopeptidase Complex
RNA, Plant / genetics
RNA, Ribosomal / genetics
Sequence Alignment
Sequence Homology, Amino Acid

Substances

Multienzyme Complexes
RNA, Plant
RNA, Ribosomal
Cysteine Endopeptidases
Proteasome Endopeptidase Complex
Adenosine Triphosphatases