The hepatitis C viral (HCV) DNA fragment coding for the core and two glycoproteins E1 + E2/NS1 were inserted into vaccinia virus vector pJSA1175, under the control of the promoter 7.5K. 143TK- cells transfected with the HCV recombinant plasmid pJSA1175CE in the presence of infectious TK+ vaccinia virus (Tiantan stain) yielded recombinant vaccinia virus and expressed an approximately 90 kDa polyprotein. Southern blot hybridization showed that the inserted HCV cDNA was located at the vaccinia virus TK+ gene, J fragment. One polypeptide band between 106 and 80 kDa was revealed in SDS-PAGE which reacted with anti-core murine serum. The polypeptide could be secreted into the medium, the total amount was about the same as in the cells.