White blood cell fragments in platelet concentrates prepared by the platelet-rich plasma or buffy-coat methods

Vox Sang. 2005 May;88(4):275-7. doi: 10.1111/j.1423-0410.2005.00634.x.

Abstract

Background and objectives: White blood cell (WBC) fragments in platelet concentrates (PCs) may induce allo-immunization in the recipient.

Materials and methods: As the level of WBC fragments can differ between PCs produced using different methods, we compared PCs prepared by using the buffy-coat method (BC-PCs) in plasma or platelet additive solution (Composol) and PCs prepared using the platelet-rich plasma method (PRP-PCs).

Results: Post-filtration results revealed identical levels of WBC, but significantly higher CD62p expression and a significantly lower amount of total DNA, cell-free DNA and number of WBC fragments (0.6 vs. 4.2 WBC equivalents/microl) in PRP-PCs than in BC-PCs in either plasma or Composol.

Conclusions: The number of WBC fragments is significantly higher in BC-PCs than in PRP-PCs.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • DNA / analysis
  • Filtration
  • Humans
  • Leukocyte Count
  • Leukocytes*
  • P-Selectin / analysis
  • Platelet Activation
  • Platelet Count
  • Plateletpheresis / methods*
  • Plateletpheresis / standards

Substances

  • P-Selectin
  • DNA