Abstract
To complement the sequencing of the three kinetoplastid genomes reported in this issue, we have undertaken a whole-organism, proteomic analysis of the four life-cycle stages of Trypanosoma cruzi. Peptides mapping to 2784 proteins in 1168 protein groups from the annotated T. cruzi genome were identified across the four life-cycle stages. Protein products were identified from >1000 genes annotated as "hypothetical" in the sequenced genome, including members of a newly defined gene family annotated as mucin-associated surface proteins. The four parasite stages appear to use distinct energy sources, including histidine for stages present in the insect vectors and fatty acids by intracellular amastigotes.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adaptation, Physiological
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Animals
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Antigens, Protozoan / analysis
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Chromatography, Liquid
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Computational Biology
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Databases, Genetic
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Energy Metabolism
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Enzymes / genetics
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Enzymes / metabolism
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Genes, Protozoan
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Genome, Protozoan
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Glycoproteins / analysis
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Glycoproteins / genetics
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Histidine / metabolism
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Life Cycle Stages
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Mass Spectrometry
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Membrane Proteins / analysis
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Membrane Proteins / genetics
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Mucins / analysis
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Multigene Family
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Neuraminidase / analysis
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Neuraminidase / genetics
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Peptides / analysis
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Protein Transport
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Proteome*
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Protozoan Proteins / analysis*
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Protozoan Proteins / genetics
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Protozoan Proteins / metabolism
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Trypanosoma cruzi / chemistry*
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Trypanosoma cruzi / genetics
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Trypanosoma cruzi / growth & development*
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Trypanosoma cruzi / metabolism
Substances
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Antigens, Protozoan
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Enzymes
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Glycoproteins
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Membrane Proteins
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Mucins
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Peptides
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Proteome
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Protozoan Proteins
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Histidine
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trans-sialidase
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Neuraminidase