Mitogen-induced expression of the primary response gene cMG1 in a rat intestinal epithelial cell-line (RIE-1)

FEBS Lett. 1992 Jul 13;306(1):1-4. doi: 10.1016/0014-5793(92)80825-2.

Abstract

cMG1 is a primary response gene first identified in a rat intestinal epithelial (RIE-1) cell-line [(1990) Oncogene 5, 1081-1083]. A number of mitogens, including epidermal growth factor (EGF), angiotensin II (AII), serum and insulin rapidly induced 2- to 6-fold increases of cMG1 mRNA in RIE-1 cells, while transforming growth factor-beta caused a small reduction. Cyclic AMP-elevating agents blocked the increase of cMG1 mRNA induced by EGF. The AII-stimulated increase of cMG1 mRNA was blocked by the depletion of protein kinase C, whereas the EGF-stimulated increase was not affected, indicating that protein kinase C-dependent and -independent signalling pathways stimulate cMG1 expression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3 Cells
  • Angiotensin II / pharmacology
  • Animals
  • Blotting, Northern
  • Butyrate Response Factor 1
  • Cell Line
  • DNA-Binding Proteins*
  • Epidermal Growth Factor / pharmacology
  • Epithelial Cells
  • Epithelium / metabolism
  • Gene Expression Regulation / drug effects*
  • Genes, fos
  • Immediate-Early Proteins*
  • Insulin / pharmacology
  • Intestinal Mucosa / metabolism*
  • Intestines / cytology
  • Mice
  • Mitogens / pharmacology*
  • Protein Kinase C / metabolism
  • Proteins / genetics*
  • RNA, Messenger / metabolism
  • Rats
  • Signal Transduction
  • Tristetraprolin
  • Tumor Cells, Cultured

Substances

  • Butyrate Response Factor 1
  • DNA-Binding Proteins
  • Immediate-Early Proteins
  • Insulin
  • Mitogens
  • Proteins
  • RNA, Messenger
  • Tristetraprolin
  • Zfp36 protein, mouse
  • Zfp36 protein, rat
  • Zfp36l1 protein, rat
  • Angiotensin II
  • Epidermal Growth Factor
  • Protein Kinase C