Toward the identification of a tolerogenic signature in IDO-competent dendritic cells

Blood. 2006 Apr 1;107(7):2846-54. doi: 10.1182/blood-2005-10-4077. Epub 2005 Dec 8.

Abstract

Although much is known about the transcriptional profiles of dendritic cells (DCs) during maturation, the molecular switches critical for the induction of a tolerogenic program in DC subsets are still obscure. We examined the gene-expression profiles of murine splenic CD8+ DCs rendered highly tolerogenic by interferon-gamma (IFN-gamma), which activates the enzyme indoleamine 2,3-dioxygenase (IDO, encoded by Indo) and thus initiates the immunosuppressive pathway of tryptophan catabolism. By examining the expression of a series of relevant genes in IDO+ compared with IDO- DCs, we found consistent and selective association of the IDO-competent phenotype with down-modulation of the Tyrobp gene, encoding the signaling adapter DAP12, which typically associates with activating receptors. Down-modulation of Tyrobp involved IFN consensus sequence binding protein (ICSBP), a transcription factor also known as IRF-8. In murine and human monocyte-derived DCs, silencing DAP12 expression imparted IDO functional competence to IDO- cells, whereas silencing IRF-8 in IDO+ counterparts abolished IDO expression and function. Thus, IRF-8 is required in tolerogenic DCs for the positive regulation of Indo and the negative regulation of Tyrobp. Overall, these studies reveal the occurrence of a simple and evolutionarily conserved code in the control of tolerance by an ancestral metabolic enzyme.

MeSH terms

  • Adaptor Proteins, Signal Transducing / genetics
  • Animals
  • DNA Primers
  • Dendritic Cells / drug effects
  • Dendritic Cells / enzymology*
  • Dendritic Cells / immunology*
  • Female
  • Gene Silencing
  • Humans
  • Immune Tolerance*
  • Indoleamine-Pyrrole 2,3,-Dioxygenase / genetics*
  • Indoleamine-Pyrrole 2,3,-Dioxygenase / metabolism
  • Interferon-gamma / pharmacology
  • Interleukin-8 / pharmacology
  • Mice
  • Mice, Inbred DBA
  • RNA, Complementary / genetics
  • Recombinant Proteins
  • Reverse Transcriptase Polymerase Chain Reaction
  • Skin Tests
  • Spleen / immunology

Substances

  • Adaptor Proteins, Signal Transducing
  • DNA Primers
  • Indoleamine-Pyrrole 2,3,-Dioxygenase
  • Interleukin-8
  • RNA, Complementary
  • Recombinant Proteins
  • Tyrobp protein, mouse
  • Interferon-gamma