Non-invasive antenatal RHD typing

Transfus Clin Biol. 2006 Mar-Apr;13(1-2):53-7. doi: 10.1016/j.tracli.2006.02.021. Epub 2006 Mar 29.

Abstract

The existence of cell free fetal DNA, derived from apoptotic syncytiotrophoblast, in the maternal circulation has opened new possibilities of non-invasive prenatal diagnosis. Although still some technical problems exists, especially the lack of a generic positive control on the presence of fetal DNA and the aspecific amplification of background maternal DNA, non-invasive prenatal RHD typing has been successfully introduced in several laboratories, especially in Europe. The diagnostic accuracy reaches>99%. In the Netherlands PCR guided administration of antenatal anti-D prophylaxis is cost-effective and nearby. In this review the main characteristics and applications of cell free fetal DNA are discussed, with an emphasis on prenatal RHD genotyping.

Publication types

  • Review

MeSH terms

  • Blood Group Incompatibility / diagnosis*
  • Blood Group Incompatibility / embryology
  • Blood Grouping and Crossmatching / economics
  • Blood Grouping and Crossmatching / methods*
  • Cost-Benefit Analysis
  • DNA / blood*
  • Erythroblastosis, Fetal / prevention & control
  • False Negative Reactions
  • False Positive Reactions
  • Female
  • Fetal Blood / immunology
  • Fetomaternal Transfusion*
  • Genotype
  • Humans
  • Polymerase Chain Reaction
  • Pregnancy
  • Prenatal Diagnosis / economics
  • Prenatal Diagnosis / methods*
  • Pseudogenes
  • Rh-Hr Blood-Group System / genetics*
  • Sensitivity and Specificity

Substances

  • Rh-Hr Blood-Group System
  • Rho(D) antigen
  • DNA