Facile synthesis and application of uniformly 13C, 15N-labeled phosphotyrosine for ligand binding studies

Adriaan W Tuin; Gregg Siegal; Gijsbert A van der Marel; Herman S Overkleeft; Dmitri V Filippov

doi:10.1016/j.bmcl.2006.04.021

Facile synthesis and application of uniformly 13C, 15N-labeled phosphotyrosine for ligand binding studies

Bioorg Med Chem Lett. 2006 Jul 15;16(14):3806-8. doi: 10.1016/j.bmcl.2006.04.021. Epub 2006 May 2.

Authors

Adriaan W Tuin¹, Gregg Siegal, Gijsbert A van der Marel, Herman S Overkleeft, Dmitri V Filippov

Affiliation

¹ Leiden Institute of Chemistry, Gorlaeus Laboratories, PO Box 9502, 2300 RA Leiden, The Netherlands.

PMID: 16650988
DOI: 10.1016/j.bmcl.2006.04.021

Abstract

The first synthesis of [U-13C, 15N] labeled phosphotyrosine is described. Preliminary studies toward the binding of phosphotyrosine to an SH2 domain have been performed by means of heteronuclear NMR.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Binding Sites
Carbon Isotopes
Isotope Labeling / methods
Ligands
Magnetic Resonance Spectroscopy / methods
Models, Chemical
Nitrogen Isotopes
Phosphotyrosine / chemical synthesis*
Phosphotyrosine / metabolism
src Homology Domains / physiology

Substances

Carbon Isotopes
Ligands
Nitrogen Isotopes
Phosphotyrosine