Comparison of PCR for sputum samples obtained by induced cough and serological tests for diagnosis of Mycoplasma pneumoniae infection in children

Clin Vaccine Immunol. 2006 Jun;13(6):708-10. doi: 10.1128/CVI.00413-05.

Abstract

Passive agglutination (PA) and immunoglobulin M (IgM), IgA, and IgG enzyme-linked immunosorbent assays (ELISAs) for the diagnosis of Mycoplasma pneumoniae were compared with PCR testing of sputum samples obtained from children with lower respiratory tract infections. The sensitivity and specificity of PA were 80.3% and 92.3% at a titer of 1:80. ELISA was found to be less sensitive than PA.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Agglutination / genetics
  • Child, Preschool
  • Cough / diagnosis
  • Cough / etiology
  • Cough / microbiology
  • Enzyme-Linked Immunosorbent Assay / methods
  • Female
  • Humans
  • Immunoglobulins / genetics
  • Immunoglobulins / metabolism
  • Infant
  • Male
  • Mycoplasma Infections / complications
  • Mycoplasma Infections / diagnosis*
  • Mycoplasma Infections / pathology
  • Mycoplasma pneumoniae / genetics
  • Mycoplasma pneumoniae / isolation & purification*
  • Serologic Tests / methods*
  • Sputum / metabolism*
  • Sputum / microbiology

Substances

  • Immunoglobulins