Physical mapping of 60 DNA markers in the p21.1----q21.3 region of the human X chromosome

Genomics. 1991 Oct;11(2):352-63. doi: 10.1016/0888-7543(91)90143-3.

Abstract

Using a panel of human/rodent somatic cell hybrids and human lymphoblast lines segregating 18 different human X-chromosome rearrangements and deletions, we have assigned 60 DNA markers to the physical map of the X chromosome from Xp21.1 to Xq21.3. Data from Southern blot hybridization and polymerase chain reaction (PCR) amplification assign these markers to 15 primary map intervals. This provides a basis for further long-range cloning and mapping of the pericentromeric region of the X chromosome.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Cell Line
  • Centromere
  • Chromosome Aberrations / genetics
  • Chromosome Mapping*
  • Chromosomes, Human
  • DNA Probes
  • Genetic Markers*
  • Humans
  • Hybrid Cells
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Polymorphism, Restriction Fragment Length
  • Tumor Cells, Cultured
  • X Chromosome*

Substances

  • DNA Probes
  • Genetic Markers