Bacterial RNA isolation with one hour centrifugation in a table-top ultracentrifuge

Biotechniques. 1990 Mar;8(3):250-1.

Abstract

A procedure for the rapid preparation of cesium-chloride purified RNA from E. coli and the cyanobacterium Synechococcus sp. PCC7942 is described. Cells are lysed in modified sucrose, Triton X-100, EDTA, Tris buffer with phenol/chloroform. The cleared lysate is extracted further with phenol/chloroform and RNA is peleted by centrifugation through a 5.7 M CsCl cushion. High quality RNA can be prepared in three hours using this procedure.

MeSH terms

  • Aurintricarboxylic Acid
  • Blotting, Northern
  • Centrifugation, Density Gradient / instrumentation
  • Centrifugation, Density Gradient / methods
  • Cyanobacteria / genetics
  • Escherichia coli / genetics
  • RNA, Bacterial / isolation & purification*
  • Ultracentrifugation / instrumentation
  • Ultracentrifugation / methods

Substances

  • RNA, Bacterial
  • Aurintricarboxylic Acid