Generating vesicular stomatitis virus pseudotype bearing the severe acute respiratory syndrome coronavirus spike envelope glycoprotein for rapid and safe neutralization test or cell-entry assay

Jinying Ge; Zhiyun Wen; Xijun Wang; Sen Hu; Yonggang Liu; Xiangang Kong; Hualan Chen; Zhigao Bu

doi:10.1196/annals.1373.030

Generating vesicular stomatitis virus pseudotype bearing the severe acute respiratory syndrome coronavirus spike envelope glycoprotein for rapid and safe neutralization test or cell-entry assay

Ann N Y Acad Sci. 2006 Oct;1081(1):246-8. doi: 10.1196/annals.1373.030.

Authors

Jinying Ge¹, Zhiyun Wen, Xijun Wang, Sen Hu, Yonggang Liu, Xiangang Kong, Hualan Chen, Zhigao Bu

Affiliation

¹ National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, P.R. China.

Abstract

We generated a recombinant vesicular stomatitis virus (VSV) pseudotype (VSV Delta G*SG) by replacing the envelope G gene with the GFP gene and complementing with spike glycoprotein (S) of SARS-CoV in trans. The neutralization and infection blocking tests showed that the VSV Delta G*SG and SARS-CoV reacted similarly to SARS-CoV specific antiserum, suggesting the VSVDelta G*SG can be a safe replacement of the live SARS-CoV for neutralization test and cell-entry assay.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Chickens
Chlorocebus aethiops
Cytopathogenic Effect, Viral
Membrane Glycoproteins / immunology*
Neutralization Tests / standards
Neutralization Tests / veterinary
Rhabdoviridae Infections / immunology
Rhabdoviridae Infections / veterinary*
Safety
Severe acute respiratory syndrome-related coronavirus / immunology*
Specific Pathogen-Free Organisms
Spike Glycoprotein, Coronavirus
Vero Cells
Vesicular stomatitis Indiana virus / genetics
Vesicular stomatitis Indiana virus / immunology*
Viral Envelope Proteins / immunology*

Substances

Membrane Glycoproteins
Spike Glycoprotein, Coronavirus
Viral Envelope Proteins