Rapid and highly sensitive pathotyping of avian influenza A H5N1 virus by using real-time reverse transcription-PCR

J Clin Microbiol. 2007 Feb;45(2):600-3. doi: 10.1128/JCM.01681-06. Epub 2006 Dec 20.

Abstract

Rapid typing of the pathogenicity of avian influenza A viruses (AIV) of subtypes H5 and H7 is crucial to initiate adequate protective measures preventing the spread of highly pathogenic AIV (HPAIV). Here, a new real-time reverse transcription-PCR assay which enables sensitive and specific detection and cleavage site analysis of HPAIV H5N1 of the Qinghai lineage is described.

Publication types

  • Evaluation Study

MeSH terms

  • Animals
  • Animals, Wild / virology
  • Base Sequence
  • Birds / virology
  • Chickens / virology
  • Hemagglutinin Glycoproteins, Influenza Virus / chemistry*
  • Hemagglutinin Glycoproteins, Influenza Virus / genetics
  • Hemagglutinin Glycoproteins, Influenza Virus / metabolism
  • Influenza A Virus, H5N1 Subtype / classification*
  • Influenza A Virus, H5N1 Subtype / genetics
  • Influenza A Virus, H5N1 Subtype / pathogenicity*
  • Influenza in Birds / epidemiology*
  • Influenza in Birds / prevention & control
  • Influenza in Birds / virology*
  • Molecular Sequence Data
  • Poultry Diseases / virology
  • Reverse Transcriptase Polymerase Chain Reaction / methods*
  • Sensitivity and Specificity
  • Time Factors
  • Turkeys / virology

Substances

  • Hemagglutinin Glycoproteins, Influenza Virus
  • hemagglutinin, avian influenza A virus