[Porcine MSC-derived CLC induced by rabbit's cardiomyocytes extract did not induce immune reaction in vivo]

Zhonghua Xin Xue Guan Bing Za Zhi. 2006 Oct;34(10):929-31.
[Article in Chinese]

Abstract

Objective: To compare the in vivo immune reaction of transplanting porcine MSC-derived CLC with rabbit cardiomyocytes extracts induced differentiation or in vitro cultured porcine MSC.

Methods: After injecting the MSC-derived CLC or MSC to the original porcines, the number of CD4+, CD8+ T cells were determined by flow cytometry. The serum concentrations of IL-2, IL-4 were measured by ELISA, and the porcine spleen lymphocyte CTL cytotoxicity was determined by Cell Counting Kit-8 Assay.

Results: The numbers of CD4+ and CD8+ T cells, the serum concentrations of IL-2, IL-4 and spleen lymphocyte CTL cytotoxicity were all similar in porcines received MSC-derived CLC induced by rabbit's CMs extract or MSC transplantation.

Conclusion: The porcine MSC-derived CLC induced by rabbit's CMs extract did not induce extra immune reaction when injected back to the original porcine.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • Bone Marrow Cells / immunology*
  • Bone Marrow Transplantation / immunology*
  • CD4 Antigens / immunology
  • CD4-CD8 Ratio
  • CD8 Antigens / immunology
  • Female
  • Male
  • Myocytes, Cardiac / cytology*
  • Rabbits
  • Swine
  • Swine, Miniature

Substances

  • Antibodies, Monoclonal
  • CD4 Antigens
  • CD8 Antigens